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KMID : 0385520130260060395
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Analytical Science & Technology
2013 Volume.26 No. 6 p.395 ~ p.400
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A solid-state NMR study on the hydration effect on the lipid phase change in the presence of an antimicrobial peptide
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Kim Chul
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Abstract
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The hydration and antimicrobial peptide effects on the lipid alignment on the surface of a thin glass plate were investigated by using the solid-state nuclear magnetic resonance spectroscopy. Pure 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphotidylcholine (POPC) lipid molecules were well aligned on the surface of a thin glass plate without direct hydration by placing the sample for a few days in the desiccator containing a saturated sodium phosphate dibasic solution, which provided 95% relative humidity. But there was a big difference between two lipid phases, the one of which was hydrated by placing the sample for a few days in the desiccator with 95% relative humidity without direct hydration and the other was directly hydrated by dropping water and then placed for a few days in the same desiccator. The surface mobility of POPC molecules in a lipid bilayer phase was much activated by water. While the time for the POPC to align on the glass plate surface was short, the time for the PG-1/POPC mixture to reach the its equilibrium state was long.
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KEYWORD
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antimicrobial peptide, protegrin-1, lipid bilayer, hydration effect, solid-state NMR,
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